Flightless I Antibody (HL2966)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48755
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2966
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human Flightless I.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Flightless I Antibody (HL2966) (NBP3-48755) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Flightless I Antibody (HL2966)
Western Blot: Flightless I Antibody (HL2966) [NBP3-48755] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Flightless I Antibody (HL2966) [NBP3-48755] -
Flightless I antibody [HL2966] detects Flightless I protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.Flightless I stained by Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: Flightless I Antibody (HL2966) [NBP3-48755] -
Flightless I antibody [HL2966] detects Flightless I protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Flightless I stained by Flightless I antibody [HL2966] (NBP3-48755) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Applications for Flightless I Antibody (HL2966)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Flightless I
Alternate Names
FLI, Fli1, flightless I homolog (Drosophila), FLILflightless I (Drosophila) homolog, MGC39265, protein flightless-1 homolog
Gene Symbol
FLII
Additional Flightless I Products
Product Documents for Flightless I Antibody (HL2966)
Product Specific Notices for Flightless I Antibody (HL2966)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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