I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Does the human beta-NGF polyclonal antibody (Catalog # AB-256-NA) cross-react with human BDNF, human NT-3, or mouse beta-NGF?

In Western blot, less than 5% cross-reactivity was observed with recombinant mouse β-NGF and recombinant rat β-NGF, and no cross-reactivity was observed with recombinant human (rh) NT-3 and rhNT-4.

What is the specificity and cross-reactivity of Catalog # AB-233-NA?

For Western blot, less than 5% cross-reactivity was observed with recombinant human (rh) β-ECGF, and no cross-reactivity was observed with rhFGF acidic, FGF-4, FGF-5, FGF-6, FGF-7, and FGF-9.

What is the heparin binding activity of FGF and VEGF?

These proteins are not assayed for their ability to bind Heparin. More information about the FGF family of growth factors is available in this review article: Basilico, C. (1992) Adv. Can. Res. 59:115.

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Does human FGF basic show activity on mouse cells?

Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

FGF basic/FGF2/bFGF Antibody [DyLight 405]

Name: FGF basic/FGF2/bFGF Antibody [DyLight 405]
Brand: Novus Biologicals
SKU: AF-233-NAE

Novus Biologicals, part of Bio-Techne | Catalog # AF-233-NAE

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AF-233-NA

FGF basic/FGF2/bFGF Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # AF-233-NAE

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