FGF acidic/FGF1 Antibody (3X3E5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16609
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3X3E5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 56-155 of human FGF acidic/FGF1 (P05230). HIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit FGF acidic/FGF1 Antibody (3X3E5) (NBP3-16609) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for FGF acidic/FGF1 Antibody (3X3E5)
![Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]](https://resources.bio-techne.com/images/products/FGF-acidic-FGF1-Antibody-3X3E5-Western-Blot-NBP3-16609-img0004.jpg "Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]")
Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609]
Western Blot: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Western blot analysis of extracts of various cell lines, using FGF acidic/FGF1 Rabbit mAb (NBP3-16609) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] -
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Confocal imaging of NIH/3T3 cells using FGF acidic/FGF1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) .The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] -
Immunocytochemistry/ Immunofluorescence: FGF acidic/FGF1 Antibody (3X3E5) [NBP3-16609] - Confocal imaging of U-2 OS cells using FGF acidic/FGF1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.Applications for FGF acidic/FGF1 Antibody (3X3E5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FGF acidic/FGF1
FGF acidic/FGF1, also known as ECGF and HBGF-1, is a secreted mitogen that stimulates the proliferation of all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It plays a number of roles in development, regeneration, and angiogenesis. FGF1 is released extracellularly as a disulfide-linked homodimer and is stored in complex with extracellular heparan sulfate. The association of FGF1 with heparan sulfate is a prerequisite for its subsequent interaction with FGF receptors. Internalized FGF1 can translocate to the cytosol and to the nucleus. Intracellular FGF1 functions as a survival factor by inhibiting p53 activity and proapoptotic signaling.
Long Name
Fibroblast Growth Factor acidic
Alternate Names
alpha-ECGF, beta-ECGF, FGF acidic, FGF-1, HBGF-1
Gene Symbol
FGF1
Additional FGF acidic/FGF1 Products
Product Documents for FGF acidic/FGF1 Antibody (3X3E5)
Product Specific Notices for FGF acidic/FGF1 Antibody (3X3E5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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