Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08984
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse
Applications
Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rat IgG2b Kappa Clone # 2.4G2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Mouse BALB/c Macrophage J774 Cell Line.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Description
Novus Biologicals Rat Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free (NBP3-08984) is a recombinant monoclonal antibody validated for use in IHC, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free
Flow Cytometry: CD16/CD32 Antibody (2.4G2) - Azide and BSA Free [NBP3-08984] -
Murine bone marrow-derived macrophages (BMDMs) were stained with anti-CD16/CD32 antibody NBP3-08984 (as well as isotype control) and bound antibody detected using goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor(R) 647(AF647) commercially available from a competitor. Compared to the isotype control and secondary-only control, a CD16/CD32-positive population is apparent.
Flow Cytometry: CD16/CD32 Antibody (2.4G2) - Azide and BSA Free [NBP3-08984] -
Ex vivo murine (C57BL6/J) monocytes and neutrophils (from zymosan-elicited peritoneal exudate, left and middle panel) as well as bone marrow-derived macrophages (BMDMs, right panel) were stained with a murine anti-human antibody (fluorescently labelled (AF647) human-specific anti-CD16) with or without pre-incubation with anti-mouse CD16/CD32 (Fc-receptors) antibody (NBP3-08984, clone 2.4G2). Pre-incubation of cells with anti-CD16/CD32 reduced non-specific binding of the fluorescently labelled antibody to all cell types analysed and shows that this anti-CD16/CD32 antibody acts as an Fc-blocking reagent.Applications for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies made by Absolute Antibody that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fc gamma RII/RIII (CD32/CD16)
Fc gamma RII/CD32 and Fc gamma RIII/CD16 proteins are low/intermediate affinity receptors for polyvalent immune-complexed IgG. Fc gamma RII is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Fc gamma RIIA associates with FcR gamma and delivers an activating signal through its cytoplasmic ITAM motif. In contrast, Fc gamma RIIB delivers an inhibitory signal through its ITIM motif. Fc gamma RIIC represents an unequal cross-over event between the IIA and IIB genes. Fc gamma RIII is expressed on NK cells, T cells, monocytes, and macrophages. It is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity, and clearance of immune complexes.
Long Name
Fc gamma Receptor II/III
Alternate Names
CD32/CD16
Gene Symbol
FCGR3A
Additional Fc gamma RII/RIII (CD32/CD16) Products
Product Documents for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free
Product Specific Notices for Fc gamma RII/RIII (CD32/CD16) Antibody (2.4G2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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