ENPP-4 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05178
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 90-230 of human ENPP4 (NP_055751.1). ANSMYDAVTKKHFSDSNDKDPFWWNEAVPIWVTNQLQENRSSAAAMWPGTDVPIHDTISSYFMNYNSSVSFEERLNNITMWLNNSNPPVTFATLYWEEPDASGHKYGPEDKENMSRVLKKIDDLIGDLVQRLKMLGLWENL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit ENPP-4 Antibody - Azide and BSA Free (NBP3-05178) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ENPP-4 Antibody - Azide and BSA Free
![Western Blot: ENPP-4 AntibodyAzide and BSA Free [NBP3-05178]](https://resources.bio-techne.com/images/products/ENPP4-Antibody-Western-Blot-NBP3-05178-img0001.jpg "Western Blot: ENPP-4 AntibodyAzide and BSA Free [NBP3-05178]")
Western Blot: ENPP-4 AntibodyAzide and BSA Free [NBP3-05178]
Western Blot: ENPP4 Antibody [NBP3-05178] - Analysis of extracts of various cell lines, using ENPP4 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitApplications for ENPP-4 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ENPP-4
ENPP-4 (Ectonucleotide pyrophosphatase/phosphodiesterase 4; NPP4) belongs to a group of ecto-enzymes which regulate the availability of extracellular nucleotides. ENPP-4 hydrolyzes phosphodiester bonds in nucleotides with a preference for adenine nucleotides. It cleaves the diadenosine compounds Ap3A and Ap4a which are released from the dense granules of thrombin-activated platelets. These reactions generate AMP and ADP from Ap3A cleavage, and AMP and ATP from Ap4A cleavage. ENPP-4 is expressed on the surface of vascular endothelial cells where its activity prolongs platelet aggretation and contributes to thrombus formation.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 4
Alternate Names
AP3Aase, ENPP4, NPP-4
Gene Symbol
ENPP4
Additional ENPP-4 Products
Product Documents for ENPP-4 Antibody - Azide and BSA Free
Product Specific Notices for ENPP-4 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov