E. Coli O157 Antibody (9.88) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54353
Conjugate
Catalog #
Key Product Details
Species Reactivity
E. coli
Applications
ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # 9.88
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A crude sonicate of E. coli O157
Reactivity Notes
E. coli O157
Localization
Whole organism
Specificity
This monoclonal antibody shows specificity to E.coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). TheE. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli(EPEC), enteroinvasiveE. coli(EIEC), enterotoxigenicE. coli(ETEC) and enterohemorrhagicE. coli(EHEC). Although the identification of pathogenicE. coli requires verification of their pathogenicity, pathogenicE. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53146).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for E. Coli O157 Antibody (9.88) - Azide and BSA Free
Application
Recommended Usage
ELISA
1 - 5 ug/ml
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: E. Coli O157
Alternate Names
H7 ompC
Additional E. Coli O157 Products
Product Documents for E. Coli O157 Antibody (9.88) - Azide and BSA Free
Product Specific Notices for E. Coli O157 Antibody (9.88) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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