E. Coli O157 Antibody (9.88)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53146
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
E. coli
Cited:
Bacteria
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
In-vitro
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Kappa Clone # 9.88
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A crude sonicate of E. coli O157
Reactivity Notes
E. coli O157
Localization
Whole organism
Specificity
This monoclonal antibody shows specificity to E.coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). TheE. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli(EPEC), enteroinvasiveE. coli(EIEC), enterotoxigenicE. coli(ETEC) and enterohemorrhagicE. coli(EHEC). Although the identification of pathogenicE. coli requires verification of their pathogenicity, pathogenicE. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54353)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for E. Coli O157 Antibody (9.88)
Application
Recommended Usage
ELISA
1-5 ug/ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
ELISA: For coating use Ab at 1-5ug/ml, order Ab without BSA.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein L or PEG purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: E. Coli O157
Alternate Names
H7 ompC
Additional E. Coli O157 Products
Product Documents for E. Coli O157 Antibody (9.88)
Product Specific Notices for E. Coli O157 Antibody (9.88)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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