dsDNA Antibody (rDSD/4565) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08466
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466] Immunocytochemistry/ Immunofluorescence: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466]](https://resources.bio-techne.com/images/products/dsDNA-Antibody-rDSD-4565-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08466-img0001.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # rDSD/4565
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Nuclei of Burkitt's cells
Localization
Nuclear
Specificity
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This monoclonal antibody produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Marker
Nuclear Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07670).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for dsDNA Antibody (rDSD/4565) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466]
Immunocytochemistry/Immunofluorescence: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466] - Immunofluorescent staining of PFA-fixed MCF-7 cells with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565) followed by goat anti-rabbit IgG-CF488 (green); phalloidin counterstain (red).Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466]
Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466] - Formalin-fixed, paraffin-embedded human lymph node stained with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565).Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466]
Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) - Azide and BSA Free [NBP3-08466] - Formalin-fixed, paraffin-embedded human colon stained with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565).Applications for dsDNA Antibody (rDSD/4565) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: dsDNA
Alternate Names
Double stranded DNA
Additional dsDNA Products
Product Documents for dsDNA Antibody (rDSD/4565) - Azide and BSA Free
Product Specific Notices for dsDNA Antibody (rDSD/4565) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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