dsDNA Antibody (rDSD/4565)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07670
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: dsDNA Antibody (rDSD/4565) [NBP3-07670] Immunocytochemistry/ Immunofluorescence: dsDNA Antibody (rDSD/4565) [NBP3-07670]](https://resources.bio-techne.com/images/products/dsDNA-Antibody-rDSD-4565-Immunocytochemistry-Immunofluorescence-NBP3-07670-img0001.jpg)
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2a Kappa Clone # rDSD/4565
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Nuclei of Burkitt's cells
Localization
Nuclear
Specificity
This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This monoclonal antibody produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Marker
Nuclear Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08466)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for dsDNA Antibody (rDSD/4565)
Immunocytochemistry/ Immunofluorescence: dsDNA Antibody (rDSD/4565) [NBP3-07670]
Immunocytochemistry/Immunofluorescence: dsDNA Antibody (rDSD/4565) [NBP3-07670] - Immunofluorescent staining of PFA-fixed MCF-7 cells with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565) followed by goat anti-rabbit IgG-CF488 (green); phalloidin counterstain (red).Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) [NBP3-07670]
Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) [NBP3-07670] - Formalin-fixed, paraffin-embedded human colon stained with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565).Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) [NBP3-07670]
Immunohistochemistry-Paraffin: dsDNA Antibody (rDSD/4565) [NBP3-07670] - Formalin-fixed, paraffin-embedded human lymph node stained with dsDNA Recombinant Mouse Monoclonal Antibody (rDSD/4565).Applications for dsDNA Antibody (rDSD/4565)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: dsDNA
Alternate Names
Double stranded DNA
Additional dsDNA Products
Product Documents for dsDNA Antibody (rDSD/4565)
Product Specific Notices for dsDNA Antibody (rDSD/4565)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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