DNMT1 Antibody

Knockout Validated: DNMT1 Antibody [NB100-264]

Knockout Validated: DNMT1 Antibody [NB100-264] - Lysates of HEK293 human embryonic kidney parental cell line and DNMT1 knockout (KO) HEK293 cell line. PVDF membrane was probed with 1:100 of Rabbit Anti-Human DNMT1 Polyclonal Antibody (Catalog # NB100-264) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for DNMT1 at approximately 240 kDa (as indicated) in the parental HEK293 cell line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.

Knockdown Validated: DNMT1 Antibody [NB100-264]

Knockdown Validated: DNMT1 Antibody [NB100-264] - Impact of extensive DNMT1 depletion in HCT116 cells on TP73 promoter methylation HCT116 human colon cancer cells have been transfected every two days for four days with the same DNMT1 siRNA (1(6)) used in KG1 cells, or exposed for 72 hours to a dose range of DAC (0, 0.001, 0.01, 0.03, 0.1, 1, 10 uM). (a) Two days after the first transfection, total proteins were extracted and subjected to Western blotting with DNMT1 and DNMT3A antibodies. Actin was used as loading control. The percentages of residual DNMT protein levels are indicated relative to the corresponding untreated controls normalized to 100%. Image collected and cropped by CiteAb from the following publication (oncotarget.com/article/3317/text/), licensed under a CC-BY license.

Simple Western: DNMT1 Antibody [NB100-264]

Simple Western: DNMT1 Antibody [NB100-264] - Image shows a specific band for Dnmt1 in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.