Key Product Details

Species Reactivity

Validated:

Human, Mouse, Non-species specific

Cited:

Human, Mouse, Rabbit

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunoassay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot, Functional Assay

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoassay, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 15A3

Format

BSA Free

Concentration

1 mg/ml

View all DNA/RNA Damage Antibodies »

Product Specifications

Immunogen

8-hydroxy-guanosine-BSA and-casein conjugates

Reactivity Notes

Use in Human reported in scientific literature (PMID:33482333) Mouse reactivity reported in scientific literature (PMID: 31226694).

Specificity

Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

Novus Biologicals Mouse DNA/RNA Damage Antibody (15A3) - BSA Free (NB110-96878) is a monoclonal antibody validated for use in IHC, ELISA, Flow, ICC/IF and IP. Anti-DNA/RNA Damage Antibody: Cited in 19 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DNA/RNA Damage Antibody (15A3) - BSA Free

Immunocytochemistry/ Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878]

Immunocytochemistry/ Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878]

Immunocytochemistry/Immunofluorescence: DNA/RNA Damage Antibody (15A3) [NB110-96878] - visualized on a retinal injury
Immunohistochemistry: DNA/RNA Damage Antibody (15A3) [NB110-96878]

Immunohistochemistry: DNA/RNA Damage Antibody (15A3) [NB110-96878]

Immunohistochemistry: DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:1000000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x. With anti-microbial.
DNA/RNA Damage Antibody (15A3) [NB110-96878] - Immunohistochemistry analysis using Mouse Anti-DNA/RNA DamageMonoclonal Antibody, Clone 15A3 (NB110-96878). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-DNA/RNA DamageMonoclonal Antibody (NB110-96878) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Applications for DNA/RNA Damage Antibody (15A3) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000
Application Notes
Use in immoprecipitation reported in scientific literature (PMID 26510519). Use in Immunoassay reported in scientific literature (PMID:31625228).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 50% Glycerol

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA/RNA Damage

DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage (1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).

Alternate Names

DNA/RNA Oxidative Damage Markers Monoclonal Antibody

Additional DNA/RNA Damage Products

Product Documents for DNA/RNA Damage Antibody (15A3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DNA/RNA Damage Antibody (15A3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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