DIAPH-2 Antibody (CL1113) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43935

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1113

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DIAPH-2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O60879, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse DIAPH-2 Antibody (CL1113) - Azide and BSA Free (NBP2-52967) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for DIAPH-2 Antibody (CL1113) - Azide and BSA Free

DIAPH-2 Antibody (CL1113) - Azide and BSA Free Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP3-43935]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP3-43935]

Staining of human kidney shows moderate positivity in renal glomeruli.
DIAPH-2 Antibody (CL1113) - Azide and BSA Free Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP3-43935]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP3-43935]

Staining of human colorectal cancer shows strong cytoplasmic immunoreactivity in tumor cells.
DIAPH-2 Antibody (CL1113) - Azide and BSA Free Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP3-43935]

Immunohistochemistry-Paraffin: DIAPH-2 Antibody [NBP3-43935]

Staining of human pancreas shows absence of immunoreactivity (negative control).
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Applications for DIAPH-2 Antibody (CL1113) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIAPH-2

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Long Name

Diaphanous Homolog 2

Alternate Names

DIA2, DIAPH2, DRF2, POF2

Gene Symbol

DIAPH2

Additional DIAPH-2 Products

Product Documents for DIAPH-2 Antibody (CL1113) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DIAPH-2 Antibody (CL1113) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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