DGAT1 Antibody (23GB5155)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51332
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB5155
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human DGAT1 (Uniprot: O75907)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DGAT1 Antibody (23GB5155)
Flow Cytometry: DGAT1 Antibody (23GB5155)[NBP3-51332] -
Flow cytometric analysis of DGAT1expression in HeLa cells using DGAT1 antibody (1:2,000). Green, isotype control; red, Diacylglycerol O-acyltransferase 1.
Flow Cytometry: DGAT1 Antibody (23GB5155)[NBP3-51332] -
Validation of DGAT1 knockdown using flow cytometry. Wild-type(WT, Blue) and DGAT1 knockdown(KD, Green) HT-1080 cells were stained with DGAT1 antibody(1:2,000) and analyzed using BD flow cytometer.
Western Blot: DGAT1 Antibody (23GB5155)[NBP3-51332] -
Western blotting analysis using DGAT1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.Applications for DGAT1 Antibody (23GB5155)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DGAT1
DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.
Long Name
Diacylglycerol O-Acyltransferase 1
Alternate Names
AGRP1, ARAT, ARGP1, DGAT, DIAR7
Gene Symbol
DGAT1
Additional DGAT1 Products
Product Documents for DGAT1 Antibody (23GB5155)
Product Specific Notices for DGAT1 Antibody (23GB5155)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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