Cytokeratin 5 Antibody (KRT5/8814R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48916

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48916-100ug
NBP3-48916-20ug
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # KRT5/8814R expressed in CHO

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 5 Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary) (Uniprot: P13647)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for Cytokeratin 5 Antibody (KRT5/8814R)

Cytokeratin 5 Antibody (KRT5/8814R)

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (KRT5/8814R) [NBP3-48916] -

Formalin-fixed, paraffin-embedded human tonsil stained with Cytokeratin 5 Antibody (KRT5/8814R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Cytokeratin 5 Antibody (KRT5/8814R)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 5

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Alternate Names

CK-5, DDD, EBS2, Keratin 5, KRT5

Gene Symbol

KRT5

Additional Cytokeratin 5 Products

Product Documents for Cytokeratin 5 Antibody (KRT5/8814R)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 5 Antibody (KRT5/8814R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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