Cytochrome b245 alpha Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13323

Novus Biologicals, part of Bio-Techne
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NBP3-13323
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Cytochrome b245 alpha Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cytochrome b245 alpha. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Cytochrome b245 alpha Antibody - BSA Free

Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323]

Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323]

Western Blot: Cytochrome b245 alpha Antibody [NBP3-13323] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Cytochrome b245 alpha antibody (NBP3-13323) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323]

Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323]

Immunohistochemistry-Paraffin: Cytochrome b245 alpha Antibody [NBP3-13323] - Cytochrome b245 alpha antibody detects Cytochrome b245 alpha protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Cytochrome b245 alpha stained by Cytochrome b245 alpha antibody (NBP3-13323) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Cytochrome b245 alpha Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytochrome b245 alpha

Alternate Names

cytochrome b light chain, Cytochrome b(558) alpha chain, cytochrome b(558) alpha-subunit, cytochrome b, alpha polypeptide, cytochrome b-245 light chain, cytochrome b-245, alpha polypeptide, Cytochrome b558 subunit alpha, flavocytochrome b-558 alpha polypeptide, Neutrophil cytochrome b 22 kDa polypeptide, p22 phagocyte B-cytochrome, p22phox, p22-PHOX, Superoxide-generating NADPH oxidase light chain subunit

Gene Symbol

CYBA

Additional Cytochrome b245 alpha Products

Product Documents for Cytochrome b245 alpha Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytochrome b245 alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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