Cyclin E2 Antibody (24GB4750)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51679
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB4750
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Cyclin E2 (Uniprot: O96020)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyclin E2 Antibody (24GB4750)
Immunocytochemistry/ Immunofluorescence: Cyclin E2 Antibody (24GB4750)[NBP3-51679] -
Immunocytochemical staining of Hela cells with Cyclin E2 antibody (1:1,000). Nuclei were stained blue with DAPI; Cyclin E2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Western Blot: Cyclin E2 Antibody (24GB4750)[NBP3-51679] -
Western blotting analysis using Cyclin E2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
Western Blot: Cyclin E2 Antibody (24GB4750)[NBP3-51679] -
Western blotting analysis using Cyclin E2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for Cyclin E2 Antibody (24GB4750)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin E2
Cyclin E2 (CCNE2) is an approximately 45-50 kDa protein that associates with the kinase Cdk2. This complex phosphorylates multiple substrates involved in regulation of cell cycle progression and the initiation of DNA replication. Cyclin E2 is required for G1/S phase progression and cell cycle reentry from G0 phase. Cyclin E2 most highly expressed in testis, thymus, and brain and is frequently upregulated in cancer. Cyclin E2 contains two cyclin box folds (aa 141-231 and aa 241-361). An alternate splice form lacks 45 amino acids within the first cyclin box fold.
Alternate Names
CCNE2, CYCE2
Gene Symbol
CCNE2
Additional Cyclin E2 Products
Product Documents for Cyclin E2 Antibody (24GB4750)
Product Specific Notices for Cyclin E2 Antibody (24GB4750)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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