Cullin 1 Antibody (23GB1555)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52041
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 23GB1555
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Cullin 1 (Uniprot: Q13616)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cullin 1 Antibody (23GB1555)
Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody (23GB1555)[NBP3-52041] -
Cullin 1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: Cullin 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cullin 1 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Immunocytochemistry/ Immunofluorescence: Cullin 1 Antibody (23GB1555)[NBP3-52041] -
Immunocytochemical staining of HT-1080 cells with Cullin 1 antibody (1:1,000). Nuclei were stained blue with DAPI; Cullin 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: Cullin 1 Antibody (23GB1555)[NBP3-52041] -
Validation of Cullin 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with Cullin 1 antibody (1:2,000) and analyzed using BD flow cytometer.Applications for Cullin 1 Antibody (23GB1555)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CUL1
Long Name
Cullin 1
Alternate Names
Cullin 1
Gene Symbol
CUL1
Additional CUL1 Products
Product Documents for Cullin 1 Antibody (23GB1555)
Product Specific Notices for Cullin 1 Antibody (23GB1555)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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