CSNK1A1/CSNK1A1L Antibody (PSH04-58)

Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]

Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - CSNK1A1/CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-33146 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33146 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: HeLa cell lysate (input)
Lane 2: NBP3-33146 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of NBP3-33146 in HeLa cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 11 seconds; ECL

Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]

Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Western blot analysis of CSNK1A1/CSNK1A1L on different lysates with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/2,000 dilution.

Lane 1: HCT 116 cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: HEK-293 cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: Jurkat cell lysate (20 ug/Lane)
Lane 6: NIH/3T3 cell lysate (20 ug/Lane)
Lane 7: RAW264.7 cell lysate (20 ug/Lane)
Lane 8: PC-12 cell lysate (20 ug/Lane)
Lane 9: Mouse brain tissue lysate (40 ug/Lane)
Lane 10: Rat brain tissue lysate (40 ug/Lane)
Lane 11: Mouse kidney tissue lysate (40 ug/Lane)
Lane 12: Rat kidney tissue lysate (40 ug/Lane)
Lane 13: Mouse spleen tissue lysate (40 ug/Lane)
Lane 14: Rat spleen tissue lysate (40 ug/Lane)

Predicted band size: 39 kDa
Observed band size: 34 kDa

Exposure time: 15 seconds; ECL;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146]

Immunohistochemistry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33146) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.