Crk Antibody (M332)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23251
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M332
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone (M332) was generated from a recombinant sequence containing the C-terminal half of mouse Crk II. This sequence has high homology to human and rat Crk II. [UniProt# Q64010]
Specificity
This antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk on SDS-PAGE immunoblots of rat PC12 and human Jurkat cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Crk Antibody (M332)
Western Blot: Mouse Crk mAb (M332) [NBP3-23251] -
Western Blot: Mouse Crk mAb (M332) [NBP3-23251] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).
Western Blot: Crk Antibody (M332) [NBP3-23251] -
Western Blot: Crk Antibody (M332) [NBP3-23251] - Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-Crk (C-terminal region) antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).Applications for Crk Antibody (M332)
Application
Recommended Usage
Immunoprecipitation
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Crk
Crk belongs to a family of adaptor molecules, which link extracellular growth and differentiation stimuli with formation of signal transduction protein complexes. Crk is mainly composed of Src homology 2 (SH2) and Src homology 3 (SH3) domains, which serve as docking sites for signalling proteins, including various receptors, cytoplasmic kinases, and GTPase regulators. These signal transduction pathways lead to modulation of cell adhesion, cell migration and immune cell responses.
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog
Alternate Names
adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)
Gene Symbol
CRK
Additional Crk Products
Product Documents for Crk Antibody (M332)
Product Specific Notices for Crk Antibody (M332)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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