Crk Antibody (3G11E8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37568
![Flow Cytometry: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]](https://resources.bio-techne.com/images/products/Crk-Antibody-3G11E8-Flow-Cytometry-NBP2-37568-img0005.jpg "Flow Cytometry: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 3G11E8
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified recombinant fragment of human Crk expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Crk Antibody (3G11E8) (NBP2-37568) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Crk Antibody (3G11E8)
![Western Blot: Crk Antibody (3G11E8)BSA Free [NBP2-37568]](https://resources.bio-techne.com/images/products/Crk-Antibody-3G11E8-Western-Blot-NBP2-37568-img0003.jpg "Western Blot: Crk Antibody (3G11E8)BSA Free [NBP2-37568]")
Western Blot: Crk Antibody (3G11E8)BSA Free [NBP2-37568]
Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate.![Immunocytochemistry/ Immunofluorescence: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]](https://resources.bio-techne.com/images/products/Crk-Antibody-3G11E8-Immunocytochemistry-Immunofluorescence-NBP2-37568-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]")
Immunocytochemistry/ Immunofluorescence: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]
Immunocytochemistry/Immunofluorescence: Crk Antibody (3G11E8) [NBP2-37568] - Immunofluorescence analysis of 3T3-L1 cells using CRK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.![Immunohistochemistry: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]](https://resources.bio-techne.com/images/products/Crk-Antibody-3G11E8-Immunohistochemistry-NBP2-37568-img0007.jpg "Immunohistochemistry: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]")
Immunohistochemistry: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]
Immunohistochemistry: Crk Antibody (3G11E8) [NBP2-37568] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues using CRK mouse mAb with DAB staining.![Western Blot: Crk Antibody (3G11E8)BSA Free [NBP2-37568]](https://resources.bio-techne.com/images/products/Crk-Antibody-3G11E8-Western-Blot-NBP2-37568-img0002.jpg "Western Blot: Crk Antibody (3G11E8)BSA Free [NBP2-37568]")
![Immunohistochemistry: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]](https://resources.bio-techne.com/images/products/Crk-Antibody-3G11E8-Immunohistochemistry-NBP2-37568-img0006.jpg "Immunohistochemistry: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]")
![ELISA: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]](https://resources.bio-techne.com/images/products/Crk-Antibody-3G11E8-ELISA-NBP2-37568-img0001.jpg "ELISA: Crk Antibody (3G11E8) - BSA Free [NBP2-37568]")
Applications for Crk Antibody (3G11E8)
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200 - 1:400
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Crk
Crk belongs to a family of adaptor molecules, which link extracellular growth and differentiation stimuli with formation of signal transduction protein complexes. Crk is mainly composed of Src homology 2 (SH2) and Src homology 3 (SH3) domains, which serve as docking sites for signalling proteins, including various receptors, cytoplasmic kinases, and GTPase regulators. These signal transduction pathways lead to modulation of cell adhesion, cell migration and immune cell responses.
Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog
Alternate Names
adapter molecule crk, avian sarcoma virus CT10 (v-crk) oncogene homolog, CRKII, p38, Proto-oncogene c-Crk, v-crk avian sarcoma virus CT10 oncogene homolog, v-crk sarcoma virus CT10 oncogene homolog (avian)
Gene Symbol
CRK
Additional Crk Products
Product Documents for Crk Antibody (3G11E8)
Product Specific Notices for Crk Antibody (3G11E8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...