COX4 Antibody (CL3515) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61620
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]](https://resources.bio-techne.com/images/products/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0001.jpg "Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]")
![Western Blot: COX4 Antibody (CL3515) [NBP2-61620]](https://resources.bio-techne.com/images/products/COX4-Antibody-CL3515-Western-Blot-NBP2-61620-img0006.jpg "Western Blot: COX4 Antibody (CL3515) [NBP2-61620]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL3515
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P13073, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Expected species cross reactivity based on sequence identity: Rat (80%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for COX4 Antibody (CL3515) - BSA Free
Western Blot: COX4 Antibody (CL3515) [NBP2-61620]
Western Blot: COX4 Antibody (CL3515) [NBP2-61620] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HeLa
Lane 3: Human cell line HEK 293
Lane 4: Human cell line A-431
Lane 5: Human cell line HepG2
![Immunocytochemistry/ Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620]](https://resources.bio-techne.com/images/products/COX4-Antibody-CL3515-Immunocytochemistry-Immunofluorescence-NBP2-61620-img0007.jpg "Immunocytochemistry/ Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620]")
Immunocytochemistry/ Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620]
Immunocytochemistry/Immunofluorescence: COX4 Antibody (CL3515) [NBP2-61620] - Staining of MCF7 cells using the Anti-COX4I1 monoclonal antibody, showing specific staining in mitochondria in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).![Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620]](https://resources.bio-techne.com/images/products/COX4-Antibody-CL3515-Immunohistochemistry-Paraffin-NBP2-61620-img0005.jpg "Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620]")
Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620]
Immunohistochemistry-Paraffin: COX4 Antibody (CL3515) [NBP2-61620] - Staining of human skin shows strong cytoplasmic immunoreactivity in epithelial cells.![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]](https://resources.bio-techne.com/images/products/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0002.jpg "Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]")
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]](https://resources.bio-techne.com/images/products/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0003.jpg "Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]")
![Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]](https://resources.bio-techne.com/images/products/COX4-Antibody-CL3515-Immunohistochemistry-NBP2-61620-img0004.jpg "Immunohistochemistry: COX4 Antibody (CL3515) [NBP2-61620]")
Applications for COX4 Antibody (CL3515) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:5000 - 1:10000
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COX4
Long Name
Cytochrome c Oxidase Subunit IV
Alternate Names
COX4I2
Gene Symbol
COX4I1
Additional COX4 Products
Product Documents for COX4 Antibody (CL3515) - BSA Free
Product Specific Notices for COX4 Antibody (CL3515) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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