Species Reactivity:

Cotton Rat

Immunogen:

NIH‑3T3 mouse embryonic fibroblast cell line transfected with cotton rat CD8 alpha and SP2/O mouse melanoma cell line transfected with cotton rat CD8 alpha.
Accession # AAL55392

Applications:

Flow Cytometry

Label:

Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)

Source:

Monoclonal Mouse IgG_2A Clone # JG12

Specificity:

Detects cotton rat CD8 alpha in direct ELISAs.

View Full Technical Details for Cotton Rat CD8 alpha PE-conjugated Antibody

Technical Data
Citations / Reviews

Applications for Cotton Rat CD8 alpha PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Cotton Rat CD8 alpha PE-conjugated Antibody

	Flow Cytometry Detection of CD8 alpha in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Mouse Anti-Cotton Rat CD8a PE-conjugated Monoclonal Antibody (Catalog # FAB7080P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD8

CD8 alpha (CD8 alpha ; also OX8, Leu-2 and Lyt-2) is a 23 kDa (predicted) member of the Ig superfamily of molecules. It is found on immature thymocytes, cytotoxic and suppressor T cells, select mast cells, and splenic and bone marrow dendritic cells (in rodent). On T cells, CD8 alpha exists as either a disulfide linked homodimer, or heterodimer bound to CD8 beta. CD8 alpha beta is best known as a coreceptor for the TCR, enhancing TCR signaling. CD8 alpha alpha serves a different function and acts as a TCR corepressor that blocks T cell activation. Based on rat, mature Sigmodon hispidus (cotton rat) CD8 alpha is a 210 amino acid (aa) type I transmembrane protein that consists of a 161 aa extracellular region (aa 24-184) with one V-type Ig-like domain (aa 35-135), and a 30 aa cytoplasmic tail (aa 206-235). Although Sigmodon hispidus is called a rat, it is not. It is a rodent, and rat CD8 alpha is the closest ortholog to cotton rat CD8 alpha currently reported. Over the extracellular region, rat and cotton rat CD8 alpha share only 54% aa identity. Rat CD8 alpha runs at 34-39 kDa in SDS-PAGE, and possesses one utilized N-linked and O-linked glycosylation site (vs. no N-linked sites in cotton rat CD8 alpha ). Based on this organization, and assuming O-linked glycosylation, cotton rat CD8 alpha would be expected to run at about 30-34 kDa in SDS-PAGE. Over aa 24-182, cotton rat CD8 alpha shares 54% and 48% aa identity with rat and mouse CD8 alpha, respectively.

Item	Value
Entrez Gene IDs	925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names	CD8, CD8 antigen, alpha polypeptide (p32), CD8A, CD8a molecule, CD_antigen: CD8a, LEU2, Leu2 T-lymphocyte antigen, MAL, OKT8 T-cell antigen, T cell co-receptor, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T-cell antigen, p32

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Cotton Rat CD8 alpha PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7080P

Key Product Details

Applications for Cotton Rat CD8 alpha PE-conjugated Antibody

Scientific Data Examples for Cotton Rat CD8 alpha PE-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: CD8

Customer Reviews for Cotton Rat CD8 alpha PE-conjugated Antibody

Product Documents for Cotton Rat CD8 alpha PE-conjugated Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

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