COMT Antibody (8E1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26646
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8E1 expressed in HEK293
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human COMT [UniProt P21964]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit COMT Antibody (8E1) - BSA Free (NBP3-26646) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for COMT Antibody (8E1) - BSA Free
Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] -
Immunohistochemistry: COMT Antibody (8E1) [NBP3-26646] - Image of COMT Antibody (8E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Western Blot: COMT Antibody (8E1) [NBP3-26646] -
Western Blot: COMT Antibody (8E1) [NBP3-26646] - Positive Western Blot detected in: 293 whole cell lysate, THP-1 whole cell lysate, HepG2 whole cell lysate.All lanes: COMT Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 31, 25 kDa
Observed band size: 31 kDa
Applications for COMT Antibody (8E1) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: COMT
Long Name
Catechol O-Methyltransferase
Alternate Names
catechol O-methyltransferase, catechol-O-methyltransferase, EC 2.1.1.6
Gene Symbol
COMT
Additional COMT Products
Product Documents for COMT Antibody (8E1) - BSA Free
Product Specific Notices for COMT Antibody (8E1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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