Coagulation Factor XIV/Protein C Antibody (3W3R7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16427

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16427-100ul
NBP3-16427-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3W3R7 expressed in HEK293

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Coagulation Factor XIV/Protein C Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-461 of human Coagulation Factor XIV/Protein C (P04070). IALLHLAQPATLSQTIVPICLPDSGLAERELNQAGQETLVTGWGYHSSREKEAKRNRTFVLNFIKIPVVPHNECSEVMSNMVSENMLCAGILGDRQDACEGDSGGPMVASFHGTWFLVGLVSWGEGCGLLHNYGVYTKVSRYLDWIHGHIRDKEAPQKSWAP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Coagulation Factor XIV/Protein C Antibody (3W3R7) (NBP3-16427) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Coagulation Factor XIV/Protein C Antibody (3W3R7)

Western Blot: Coagulation Factor XIV/Protein C Antibody (3W3R7) [NBP3-16427]

Western Blot: Coagulation Factor XIV/Protein C Antibody (3W3R7) [NBP3-16427]

Western Blot: Coagulation Factor XIV/Protein C Antibody (3W3R7) [NBP3-16427] - Western blot analysis of extracts of various cell lines, using Coagulation Coagulation Factor XIV/Protein C Rabbit mAb (NBP3-16427) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Coagulation Factor XIV/Protein C Antibody (3W3R7)

Immunocytochemistry/ Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (3W3R7) [NBP3-16427] -

Immunocytochemistry/ Immunofluorescence: Coagulation Factor XIV/Protein C Antibody (3W3R7) [NBP3-16427] - Confocal imaging of paraffin-embedded Mouse liver tissue using Coagulation Factor XIV/Protein C Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for Coagulation Factor XIV/Protein C Antibody (3W3R7)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Coagulation Factor XIV/Protein C

Protein C is a vitamin K-dependent serine protease that is produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin bound to an endothelial surface receptor and the active enzyme cleaves factor Va and VIIIa inhibiting blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentration in human plasma is approximately 1-3 ng/ml while the concentration of the proenzyme is approximately 3 ug/mL due to the very fast turnover.

Long Name

Vitamin K-dependent Protein C

Alternate Names

Autoprothrombin IIA, PROC, Protein C

Gene Symbol

PROC

Additional Coagulation Factor XIV/Protein C Products

Product Documents for Coagulation Factor XIV/Protein C Antibody (3W3R7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Coagulation Factor XIV/Protein C Antibody (3W3R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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