CNOT1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92464
Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2137-2376 of human CNOT1 (NP_057368.3). PRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CNOT1 Antibody - Azide and BSA Free
![Western Blot: CNOT1 AntibodyAzide and BSA Free [NBP2-92464]](https://resources.bio-techne.com/images/products/CNOT1-Antibody-Western-Blot-NBP2-92464-img0003.jpg "Western Blot: CNOT1 AntibodyAzide and BSA Free [NBP2-92464]")
Western Blot: CNOT1 AntibodyAzide and BSA Free [NBP2-92464]
Western Blot: CNOT1 Antibody [NBP2-92464] - Whole cell lysate from SUM159 cells was loaded with 50 ug/lane. 10% SDS-PAGE. CNOT1 Antibody (NBP2-92464) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.![Western Blot: CNOT1 AntibodyAzide and BSA Free [NBP2-92464]](https://resources.bio-techne.com/images/products/CNOT1-Antibody-Western-Blot-NBP2-92464-img0001.jpg "Western Blot: CNOT1 AntibodyAzide and BSA Free [NBP2-92464]")
Western Blot: CNOT1 AntibodyAzide and BSA Free [NBP2-92464]
Western Blot: CNOT1 Antibody [NBP2-92464] - Analysis of extracts of various cell line, using CNOT1 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: CNOT1 Antibody - Azide and BSA Free [NBP2-92464] -
Immunocytochemistry/ Immunofluorescence: CNOT1 Antibody - Azide and BSA Free [NBP2-92464] - Immunofluorescence analysis of L929 cells using CNOT1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for CNOT1 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:200-1:2000
Reviewed Applications
Read 1 review rated 5 using NBP2-92464 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CNOT1
Alternate Names
AD-005, adrenal gland protein AD-005, CCR4-associated factor 1, CCR4-NOT transcription complex subunit 1, CCR4-NOT transcription complex, subunit 1, CDC39DKFZp686E0722, hNOT1, KIAA1007DKFZp686O168, Negative regulator of transcription subunit 1 homolog, NOT1 (negative regulator of transcription 1, yeast) homolog, NOT1FLJ90644, NOT1HFLJ36492
Gene Symbol
CNOT1
Additional CNOT1 Products
Product Documents for CNOT1 Antibody - Azide and BSA Free
Product Specific Notices for CNOT1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov
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