CLEC9a Antibody (8F9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07340
Clone 8F9 was used by HLDA to establish CD designation.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 8F9
Concentration
0.2 mg/ml
Product Specifications
Immunogen
RBL-2H3 cells expressing human CLEC9a fused to an HA epitope
Epitope
aa 50-110
Localization
Cell Surface
Specificity
This monoclonal antibody recognizes a protein of 27kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor.
Marker
Dendritic Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08246)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for CLEC9a Antibody (8F9)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA).
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CLEC9a
Long Name
C-type Lectin-like Receptor 9
Alternate Names
CD370, DNGR1
Gene Symbol
CLEC9A
UniProt
Additional CLEC9a Products
Product Documents for CLEC9a Antibody (8F9)
Product Specific Notices for CLEC9a Antibody (8F9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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