CKAP2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84602

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NBP1-84602
NBP1-84602-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CKAP2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPIRSHHSNTRDTVKQGISRTSANVTIRKGPHEKELLQSKTALSSVKTSSSQGIIRNKTLSRSIASEVVARPASLSNDKLMEKSEPVDQRRHTAGKAIVDSRSAQPKETSEERKARLSEWKAGKGR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CKAP2 Antibody - BSA Free

CKAP2 Antibody - BSA Free Immunohistochemistry-Paraffin: CKAP2 Antibody - BSA Free [NBP1-84602]

Immunohistochemistry-Paraffin: CKAP2 Antibody - BSA Free [NBP1-84602]

Analysis in human testis and pancreas tissues using HPA008410 antibody. Corresponding CKAP2 RNA-seq data are presented for the same tissues.
CKAP2 Antibody - BSA Free Immunohistochemistry-Paraffin: CKAP2 Antibody - BSA Free [NBP1-84602]

Immunohistochemistry-Paraffin: CKAP2 Antibody - BSA Free [NBP1-84602]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
CKAP2 Antibody - BSA Free Immunohistochemistry-Paraffin: CKAP2 Antibody - BSA Free [NBP1-84602]

Immunohistochemistry-Paraffin: CKAP2 Antibody - BSA Free [NBP1-84602]

Staining of human tonsil shows moderate membranous cytoplasmic positivity in germinal center cells.
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Applications for CKAP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CKAP2

CKAP2 (cytoskeletal-associated protein 2), also known as TMAP (tumor-associated microtubule-associated protein), was identified as an mRNA up-regulated in primary human gastric cancers. It was found to localize to microtubule organizing centers and microtubules and play a role in cell division. CKAP2 is required for proper chromosome segregation and the maintenance of genomic stability. It has recently been shown to be a substrate of Cdk1-cyclinB and essential for bipolar spindle formation.

Alternate Names

CTCL tumor antigen se20-10, cytoskeleton associated protein 2, cytoskeleton-associated protein 2, LB1DKFZp686L1238, se20-10, TMAPFLJ10749, Tumor- and microtubule-associated protein, tumor-associated microtubule-associated protein

Gene Symbol

CKAP2

Additional CKAP2 Products

Product Documents for CKAP2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CKAP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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