Chromogranin A Antibody (CL0166) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52869
![Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-CL0166-Immunohistochemistry-Paraffin-NBP2-52869-img0001.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]")
![Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-CL0166-Immunohistochemistry-Paraffin-NBP2-52869-img0003.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]")
![Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-CL0166-Western-Blot-NBP2-52869-img0004.jpg "Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0166
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This antibody was developed using a recombinant protein derived from P10645, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Chromogranin A Antibody (CL0166) - BSA Free
Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869]
Western Blot: Chromogranin A Antibody (CL0166) [NBP2-52869] - Lane 1: Marker [kDa] Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)![Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-CL0166-Immunocytochemistry-Immunofluorescence-NBP2-52869-img0006.jpg "Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869]")
Immunocytochemistry/ Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869]
Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody (CL0166) [NBP2-52869] - staining of SH-SY 5Y cells using the Anti-CHGA monoclonal antibody, showing specific staining in vesicles in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.![Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-CL0166-Immunohistochemistry-Paraffin-NBP2-52869-img0002.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]")
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869]
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CL0166) [NBP2-52869] - Staining of human duodenum shows strong positivity in a subset of enteroendocrine cells.Applications for Chromogranin A Antibody (CL0166) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Chromogranin A
Alternate Names
Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I
Gene Symbol
CHGA
Additional Chromogranin A Products
Product Documents for Chromogranin A Antibody (CL0166) - BSA Free
Product Specific Notices for Chromogranin A Antibody (CL0166) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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