CHIP/STUB1 Antibody (23GB2345)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51037

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NBP3-51037-100ul
NBP3-51037-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2345

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CHIP/STUB1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human CHIP/STUB1 (Uniprot: Q9UNE7)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CHIP/STUB1 Antibody (23GB2345)

CHIP/STUB1 Antibody (23GB2345)

Immunocytochemistry/ Immunofluorescence: CHIP/STUB1 Antibody (23GB2345)[NBP3-51037] -

Immunocytochemical staining of C2C12 cells with CHIP/STUB1 antibody (1:1,000). Nuclei were stained blue with DAPI; CHIP/STUB1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
CHIP/STUB1 Antibody (23GB2345)

Flow Cytometry: CHIP/STUB1 Antibody (23GB2345)[NBP3-51037] -

Flow cytometric analysis of CHIP/STUB1expression in C2C12 cells using CHIP/STUB1 antibody (1:2,000). Green, isotype control; red, STUB1.
CHIP/STUB1 Antibody (23GB2345)

Western Blot: CHIP/STUB1 Antibody (23GB2345)[NBP3-51037] -

Western blotting analysis using CHIP/STUB1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.
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Applications for CHIP/STUB1 Antibody (23GB2345)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CHIP/STUB1

STUB1/CHIP is an E3 ubiquitin ligase and co-chaperone of heat-shock protein 70 (Hsp70). STUB1/CHIP interacts with Hsp70 to monitor protein folding and direct misfolded proteins for proteasomal degradation. It has been found to also associate with Hsp90 to remove phosphorylated protein tau, a protein that accumulates in the brains of Alzheimers and Down's syndrome patients. STUB1/CHIP has been reported to also have intrinsic chaperone activity and recognize non-native proteins in response to heat stress. In this way, STUB1/CHIP has been shown to be a direct chaperone for p53. Alternate names for STUB1/CHIP include carboxy terminus of Hsp70-interacting protein, STIP1 homology and U-box containing protein 1, CLL-associated antigen KW-8 antigen, serologically defined colon cancer antigen 7, heat shock protein A binding protein 2, NY-CO-7, HSPABP2, and SDCCAG7.

Long Name

C-terminus of Hsc70 Interacting Protein

Alternate Names

HSPABP2, NY-CO-7, SDCCAG7, STUB1, UBOX1

Gene Symbol

STUB1

Additional CHIP/STUB1 Products

Product Documents for CHIP/STUB1 Antibody (23GB2345)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CHIP/STUB1 Antibody (23GB2345)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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