Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all CEBP Beta Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 50 and 100 of human CCAAT/Enhancer Binding Protein (C/EBP), beta using the numbering given in entry NP_005185.2 (GeneID 1051).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CEBP Beta Antibody - BSA Free

Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179] - Detection of Human C/EBP beta by Western Blot. Samples: Whole cell lysate from HeLa (50, 15, 5 ug), 293T (50 ug), and Jurkat (50 ug) cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody NBP1-46179 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179]

Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179]

Immunohistochemistry-Paraffin: CEBP Beta Antibody [NBP1-46179] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-C/EBP beta used at a dilution of 1:1,000 (1ug/ml) Detection: DAB staining using Immunohistochemistry Accessory Kit .
Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179]

Western Blot: CEBP Beta Antibody [NBP1-46179] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-C/EBP beta antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. C/EBP beta was also immunoprecipitated by rabbit anti-C/EBP beta antibody NBP1-46180, which recognizes a downstream epitope.

Applications for CEBP Beta Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CEBP Beta

NF-IL6 is a member of the CAAT/enhancer-binding protein (C/EBP) family of transcription factors. It has been shown to directly interact with the retinoblastoma (RB) protein.

Alternate Names

C/EBP beta, C/EBP-beta, CCAAT/enhancer binding protein (C/EBP), beta, CCAAT/enhancer-binding protein beta, CRP2, IL6DBP, interleukin 6-dependent DNA-binding protein, LAPTCF-5, Liver activator protein, liver-enriched transcriptional activator protein, MGC32080, NF-IL6, Nuclear factor NF-IL6, nuclear factor of interleukin 6, TCF5NFIL6, Transcription factor 5

Gene Symbol

CEBPB

Additional CEBP Beta Products

Product Documents for CEBP Beta Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CEBP Beta Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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