Cdx1 Antibody (11E10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26662

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26662-100ul
NBP3-26662-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 11E10 expressed in HEK293

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CDX1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Cdx1 [UniProt P47902]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Cdx1 Antibody (11E10) - BSA Free (NBP3-26662) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Cdx1 Antibody (11E10) - BSA Free

Cdx1 Antibody (11E10)

Western Blot: Cdx1 Antibody (11E10) [NBP3-26662] -

Western Blot: Cdx1 Antibody (11E10) [NBP3-26662] - Positive Western Blot detected in: HepG2 whole cell lysate.
All lanes: Cdx1 Antibody at 1:2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 29, 15 kDa
Observed band size: 25-30 kDa
Cdx1 Antibody (11E10)

Immunocytochemistry/Immunofluorescence: Cdx1 Antibody (11E10) [NBP3-26662] -

Immunocytochemistry/Immunofluorescence: Cdx1 Antibody (11E10) [NBP3-26662] - Staining of Hela cell with Cdx1 Antibody (11E10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 524-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Cdx1 Antibody (11E10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: CDX1

The transcription factors Cdx1 and Cdx2 are members of the caudal-related homeobox gene family based on their homology to the factor caudal in Drosophila melanogaster. The caudal homeobox factor is required for anterior-posterior regional identity and axial patterning in Drosophila. During mouse development, the expression of Cdx1 and Cdx2 can be divided into two stages that likely reflect distinct roles. During early development, these two factors are expressed in a Hox-like manner in the three germ layers and appear to have overlapping functions in anterior-posterior patterning and posterior axis elongation. The second stage of Cdx1 and Cdx2 expression, from late development into adulthood, is characterized by the loss of expression almost everywhere but the epithelium of small intestine and colon. Putative intestine-specific enhancers upstream of the CDX1 gene have been identified that may orchestrate the switch in expression patterns for that gene. As might be expected from this pattern of expression, a wide variety of in vivo and in vitro studies have suggested that these two factors are important in controlling epithelial cell differentiation, proliferation, and function in the intestine.

Long Name

Caudal-type Homeobox Transcription Factor 1

Alternate Names

caudal type homeo box transcription factor 1, caudal type homeobox 1, caudal type homeobox transcription factor 1, Caudal-type homeobox protein 1, caudal-type homeobox protein CDX1, homeobox protein CDX-1, MGC116915

Gene Symbol

CDX1

Additional CDX1 Products

Product Documents for Cdx1 Antibody (11E10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Cdx1 Antibody (11E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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