Immunocytochemistry/Immunofluorescence: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of RH-30 cells using the Anti-CDK4 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912]

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912] - Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.

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Applications for CDK4 Antibody (CL8102) - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml

Application Notes

For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK4

Cdk4 is encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Long Name

Cyclin-dependent Kinase 4

Alternate Names

CMM3, PSK-J3

Gene Symbol

CDK4

Additional CDK4 Products

Product Documents for CDK4 Antibody (CL8102) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CDK4 Antibody (CL8102) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CDK4 Antibody (CL8102) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88912

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Format

Concentration

View all CDK4 Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Scientific Data Images for CDK4 Antibody (CL8102) - BSA Free

Western Blot: CDK4 Antibody (CL8102) [NBP2-88912]

Immunocytochemistry/ Immunofluorescence: CDK4 Antibody (CL8102) [NBP2-88912]

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912]

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912]

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912]

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912]

Immunohistochemistry-Paraffin: CDK4 Antibody (CL8102) [NBP2-88912]

Applications for CDK4 Antibody (CL8102) - BSA Free

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CDK4

Long Name

Alternate Names

Gene Symbol

Additional CDK4 Products

Product Documents for CDK4 Antibody (CL8102) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CDK4 Antibody (CL8102) - BSA Free