CD99 Antibody (CL12929) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44371

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL12929

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD99 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P14209, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CD99 Antibody (CL12929) - Azide and BSA Free

CD99 Antibody (CL12929) - Azide and BSA Free Immunohistochemistry-Paraffin: CD99 Antibody [NBP3-44371]

Immunohistochemistry-Paraffin: CD99 Antibody [NBP3-44371]

Staining of human glioma shows moderate membranous positivity in tumor cells.
CD99 Antibody (CL12929) - Azide and BSA Free Immunohistochemistry-Paraffin: CD99 Antibody [NBP3-44371]

Immunohistochemistry-Paraffin: CD99 Antibody [NBP3-44371]

Staining of human kidney shows no positivity in cells in tubules as expected.
CD99 Antibody (CL12929) - Azide and BSA Free Immunohistochemistry-Paraffin: CD99 Antibody [NBP3-44371]

Immunohistochemistry-Paraffin: CD99 Antibody [NBP3-44371]

Staining of human pancreas shows strong membranous positivity in islets of Langerhans.
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Applications for CD99 Antibody (CL12929) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD99

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CD99, MIC2, pilr-1, PILR-L

Gene Symbol

CD99

Additional CD99 Products

Product Documents for CD99 Antibody (CL12929) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD99 Antibody (CL12929) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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