CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20165
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Cell depletion, ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # PK-136
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing (C3H X BALB)F1 mice with spleen cells enriched for NK-1 cells and bone marrow cells from CE mice.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free
Immunocytochemistry /Immunofluorescence: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] -
Immunocytochemistry /Immunofluorescence: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with NBP3-20165 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20165, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.
Flow Cytometry: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] -
Flow Cytometry: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-20165 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] -
Western Blot: CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free [NBP3-20165] - RAW264.7(A) (1ug/ml) cell lysate, mouse spleen(B) (1ug/ml) and mouse lymph node(C) (0.3ug/ml) tissue lysate (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with NBP3-20165 before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.Applications for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free
Application
Recommended Usage
Cell depletion
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD161/NK1.1
Alternate Names
Klrb1c, Ly-59, Ly55c
Gene Symbol
Klrb1c
Additional CD161/NK1.1 Products
Product Documents for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free
Product Specific Notices for CD161/NK1.1 Antibody (PK-136) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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