Caspase 5 Antibody (SR3060) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27741

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-27741-100ul
NBP3-27741-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR3060 expressed in HEK293

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Caspase-5 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Caspase 5 (Uniprot #: P51878)

Specificity

Detects endogenous levels of Caspase 5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Caspase 5 Antibody (SR3060) - BSA Free (NBP3-27741) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Caspase 5 Antibody (SR3060) - BSA Free

Immunohistochemistry-Paraffin: Caspase 5 Antibody (SR3060) [NBP3-27741] -

Immunohistochemistry-Paraffin: Caspase 5 Antibody (SR3060) [NBP3-27741] - Immunohistochemical analysis of paraffin-embedded human breast cancer.

Western Blot: Caspase 5 Antibody (SR3060) [NBP3-27741] -

Western Blot: Caspase 5 Antibody (SR3060) [NBP3-27741] - Western blot analysis of Caspase 5 expression in HeLa cell lysate.

Applications for Caspase 5 Antibody (SR3060) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Caspase-5

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-5 (ICH-3, ICEREL-III, TY protease) is a member of the caspase-1 subfamily and it is an upstream mediator of apoptosis. Caspase-5 is highly expressed in lung, liver, and skeletal muscle, and similar to Caspase-4, its overexpression can induce cellular apoptosis (1). A tetramer, Caspase-5 separates into two subunits of a 20kda (p20) and 10kda (p10) after activation. In vitro, Caspase-5 can be cleaved by GranB, a major player in Cytotoxic T lymphocytes (CTL) induced apoptosis (2). In vivo, the Caspase-5 expression is elevated by LPS and IFNg. Caspase-5 induced apoptosis can be inhibited by CrmA and Bcl-2. Caspase-5 has also been implicated in cellular inflammatory response and in the microsatelite mutator pathway for cancer (3).

Alternate Names

CASP5, Caspase5, ICE(rel)III, Protease ICH-3, Protease TY

Gene Symbol

CASP5

Additional Caspase-5 Products

Product Documents for Caspase 5 Antibody (SR3060) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Caspase 5 Antibody (SR3060) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.