Recombinant Canine VEGF (Catalog # 1603-CV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine VEGF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine VEGF₁₆₄Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603). The ND₅₀ is typically 0.04-0.10 µg/mL.

VEGF in Canine PBMCs.

VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Canine VEGF ELISA Standard Curve

Recombinant Canine VEGF (Catalog # 1603-CV) was serially diluted and captured by Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Canine VEGF₁₆₄ Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed canine peripheral blood mononuclear cells

Western Blot

0.1 µg/mL
Sample: Recombinant Canine VEGF (Catalog # 1603-CV)

Neutralization

Measured by its ability to neutralize VEGF-induced proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 0.04-0.10 µg/mL in the presence of 15 ng/mL Recombinant Canine VEGF.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Canine VEGF164 is an approximately 45 kDa molecular weight homodimer Canine sharing 91%, 90%, and 98% aa sequence identity with the rat, mouse, and feline VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Alternate Names

MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Gene Symbol

VEGFA

UniProt

Q9MYV3

Additional VEGF Products

Product Documents for Canine VEGF₁₆₄ Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Canine VEGF₁₆₄ Antibody

For research use only

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Canine VEGF164 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF1603

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all VEGF Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Canine VEGF164 Antibody

Cell Proliferation Induced by VEGF and Neutralization by Canine VEGF Antibody.