Calretinin Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32244
![Western Blot: Calretinin Antibody [NBP1-32244]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Western-Blot-NBP1-32244-img0005.jpg "Western Blot: Calretinin Antibody [NBP1-32244]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Predicted:
Bovine (97%), Porcine (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (89%).
Marker
Mesothelial Cell Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Calretinin Antibody - BSA Free (NBP1-32244) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Calretinin Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Calretinin Antibody - BSA Free
![Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP1-32244]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32244-img0010.jpg "Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP1-32244]")
Immunocytochemistry/ Immunofluorescence: Calretinin Antibody [NBP1-32244]
Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-32244] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.![Western Blot: Calretinin Antibody [NBP1-32244]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Western-Blot-NBP1-32244-img0008.jpg "Western Blot: Calretinin Antibody [NBP1-32244]")
Western Blot: Calretinin Antibody [NBP1-32244]
Western Blot: Calretinin Antibody [NBP1-32244] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CALB2-transfected 293T cells.![Western Blot: Calretinin Antibody [NBP1-32244]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Western-Blot-NBP1-32244-img0015.jpg "Western Blot: Calretinin Antibody [NBP1-32244]")
Western Blot: Calretinin Antibody [NBP1-32244]
Western Blot: Calretinin Antibody [NBP1-32244] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-32244-img0007.jpg "Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244]")
![Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244]](https://resources.bio-techne.com/images/products/Calretinin-Antibody-Immunohistochemistry-Paraffin-NBP1-32244-img0016.jpg "Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244]")
Applications for Calretinin Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Frozen
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Calretinin
Alternate Names
CAB29, CAL2, CALB2
Gene Symbol
CALB2
Additional Calretinin Products
Product Documents for Calretinin Antibody - BSA Free
Product Specific Notices for Calretinin Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.