Calnexin Antibody (1C2.2D11) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36570F
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (91%), Canine (92%), Primate (99%), Rat (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 1C2.2D11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Partial recombinant human Calnexin protein (between amino acids 1-300). [UniProt P27824]
Reactivity Notes
Immunogen's sequence similarity with other species: Mouse (88%), Duck (81%), Xenopus (76%)
Localization
Endoplasmic reticulum membrane, Melanosome
Marker
Endoplasmic Reticulum Membrane Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
Scientific Data Images for Calnexin Antibody (1C2.2D11) [FITC]
Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [FITC] [NBP2-36570F] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Calnexin (1C2.2D11) conjugated to FITC [NBP2-36570F] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): Calnexin Antibody (1C2.2D11) [FITC] [NBP2-36570F] - An intracellular stain was performed on HeLa cells with Calnexin Antibody (1C2.2D11) NBP2-36570F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
![Calnexin Antibody (1C2.2D11) [FITC]](https://resources.bio-techne.com/images/products/nbp2-36570f_mouse-monoclonal-calnexin-antibody-1c2-2d11-fitc-immunocytochemistry-immunofluorescence-142025121141..jpg "Calnexin (1C2.2D11) in U-2 OS Human Cell Line.")
Calnexin (1C2.2D11) in U-2 OS Human Cell Line.
Calnexin (1C2.2D11) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-Calnexin (1C2.2D11) Protein G Purified Monoclonal Antibody conjugated to FITC (Catalog # NBP2-36570F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.Applications for Calnexin Antibody (1C2.2D11) [FITC]
Application
Recommended Usage
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-36570. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Calnexin
Alternate Names
calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, P90
Gene Symbol
CANX
Additional Calnexin Products
Product Documents for Calnexin Antibody (1C2.2D11) [FITC]
Product Specific Notices for Calnexin Antibody (1C2.2D11) [FITC]
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...