Western Blot: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of 11 kDa Partial Recombinant Human Calnexin protein with Calnexin antibody (clone 1C2.2D11) at 0.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Calnexin (1C2.2D11) conjugated to FITC [NBP2-36570F] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human kidney using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cells of Glomeruli developed strong cytoplasmic staining.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin [1C2.2D11] Antibody NBP2-36570AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Calnexin (1C2.2D11) antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of normal human breast using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The myoepithelial cells around the lobules depicted a very strong cytoplasmic staining.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of malignant stromal tumor of the human small bowel using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The cancer cells showed a very strong cytoplasmic reactivity for Calnexin.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human placenta using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration.

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) [NBP2-36570] - Analysis of FFPE tissue section of human skin using mouse monoclonal Calnexin antibody (clone 1C2.2D11) at 7 ug/mL concentration. The outermost keratinocytes layer of the epidermis showed cytoplasmic positivity for Calnexin protein.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - Intracellular staining of human Calnexin in Flow cytometry using 5.0 ug of antibody per 1 million cells. Isotype control was mouse IgG2b kappa.

Flow (Intracellular): Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Flow (Intracellular): Calnexin Antibody (1C2.2D11) [NBP2-36570] - An intracellular stain was performed on HeLa cells with Calnexin Antibody (1C2.2D11) NBP2-36570F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

Calnexin (IC2.2D11) in U-2 OS Human Cell Line.

Calnexin (IC2.2D11) was detected in immersion U-2 OS human osteosarcoma cell line using Mouse anti-Calnexin (IC2.2D11) Protein G Purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP2-36570AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (dark blue). Cells were imaged using a 100X objective and digitally deconvolved.

Calnexin (1C2.2D11) in U-2 OS Human Cell Line.

Calnexin (1C2.2D11) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-Calnexin (1C2.2D11) Protein G Purified Monoclonal Antibody conjugated to FITC (Catalog # NBP2-36570F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

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Applications for Calnexin Antibody (1C2.2D11) - BSA Free

Application

Recommended Usage

Flow (Intracellular)

5 ug/million cells

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

7 ug/ml

Immunohistochemistry-Paraffin

7 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calnexin

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, P90

Entrez Gene IDs

821 (Human); 12330 (Mouse); 29144 (Rat)

Gene Symbol

CANX

UniProt

P27824 (Human)

Additional Calnexin Products

Product Documents for Calnexin Antibody (1C2.2D11) - BSA Free

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download ICC/IF Protocol

Download IHC-P Protocol

Download Western Blot Protocol

COA

SDS

Download SDS

Product Specific Notices for Calnexin Antibody (1C2.2D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Calnexin Antibody (1C2.2D11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36570

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all Calnexin Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Marker

Clonality

Host

Isotype

Scientific Data Images for Calnexin Antibody (1C2.2D11) - BSA Free

Western Blot: Calnexin Antibody (1C2.2D11)BSA Free [NBP2-36570]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Flow (Intracellular): Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunocytochemistry/ Immunofluorescence: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Immunohistochemistry-Paraffin: Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Flow (Intracellular): Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Flow (Intracellular): Calnexin Antibody (1C2.2D11) - BSA Free [NBP2-36570]

Calnexin (IC2.2D11) in U-2 OS Human Cell Line.

Calnexin (1C2.2D11) in U-2 OS Human Cell Line.

Applications for Calnexin Antibody (1C2.2D11) - BSA Free

Flow (Intracellular)

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: Calnexin

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional Calnexin Products

Product Documents for Calnexin Antibody (1C2.2D11) - BSA Free

Product Datasheets

Protocols & Manuals

COA

Certificate of Analysis

SDS

Product Specific Notices for Calnexin Antibody (1C2.2D11) - BSA Free