Bub1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31584
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Bub1. The exact sequence is proprietary.
Reactivity Notes
Bovine (80%).
Localization
Nucleus, Kinetochore
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Bub1 Antibody - BSA Free
![Western Blot: Bub1 Antibody [NBP1-31584]](https://resources.bio-techne.com/images/products/BUB1-Antibody-Western-Blot-NBP1-31584-img0003.jpg "Western Blot: Bub1 Antibody [NBP1-31584]")
Western Blot: Bub1 Antibody [NBP1-31584]
Western Blot: BUB1 Antibody [NBP1-31584] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:3000.
Western Blot: Bub1 Antibody [NBP1-31584] -
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Bub1 antibody (NBP1-31584) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Bub1 Antibody [NBP1-31584] -
Bub1 antibody detects Bub1 protein by western blot analysis.A. 30 ug 293T whole cell lysate/extract
5 % SDS-PAGE
Bub1 antibody (NBP1-31584) dilution: 1:1000
Applications for Bub1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Bub1
Long Name
BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog
Alternate Names
BUB1 budding uninhibited by benzimidazoles 1 homolog, BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1, BUB1L, budding uninhibited by benzimidazoles 1 (yeast homolog), budding uninhibited by benzimidazoles 1 homolog (yeast), EC 2.7.11.1, hBUB1mitotic spindle checkpoint kinase, putative serine/threonine-protein kinase
Gene Symbol
BUB1
Additional Bub1 Products
Product Documents for Bub1 Antibody - BSA Free
Product Specific Notices for Bub1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.