BRCC3 Antibody - BSA Free

Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] -

Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat deltaK (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] -

Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat deltaK (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: BRCC3 Antibody - BSA Free [NBP1-76831] -

Immunohistochemistry: BRCC3 Antibody - BSA Free [NBP1-76831] - Immunohistochemistry of BRCC3 in human brain tissue with BRCC3 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody - BSA Free [NBP1-76831] -

Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody - BSA Free [NBP1-76831] - Immunofluorescence of BRCC3 in Human Brain cells with BRCC3 antibody at 20 ug/mL.