BRCA1 [p Ser1423] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-226

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Monkey (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all BRCA1 Antibodies »

Product Specifications

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Breast Cancer Gene 1 (GeneID 672) around serine 1423 according to the numbering given in entry NP_009225.1.

Reactivity Notes

Gorilla (100%), Chimpanzee (100%).

Modification

p Ser1423

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit BRCA1 [p Ser1423] Antibody - BSA Free (NB100-226) is a polyclonal antibody validated for use in WB and Flow. Anti-BRCA1 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BRCA1 [p Ser1423] Antibody - BSA Free

Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226]

Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226]

Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226] - Detection of Human Phospho BRCA1 (S1423) by Western Blot. Samples : Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1423) antibody NB100-226 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226]

Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226]

Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226] - Detection of Brca1 phospho Ser 1423 by Western blot using NB 100-226. Sample whole cell lysate (25 mcg/lane) from HeLa cells treated with 10 Gy IR or untreated. NB 100-226 was used at 1:2500 dilution.

Applications for BRCA1 [p Ser1423] Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Western Blot

1:1000-1:10000
Application Notes
Use in FLOW cytometry reported in scientific literature ( PMID: 24965603).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRCA1

BRCA 1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA 1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA 1 function confers an increased susceptibility for both breast and ovarian cancer.

Long Name

Breast Cancer 1

Alternate Names

BRCAI, breast and ovarian cancer susceptibility protein 1, breast and ovarian cancer sususceptibility protein, breast cancer 1, early onset, breast cancer type 1 susceptibility protein, EC 6.3.2, EC 6.3.2.-, IRIS, PNCA4, PSCP, subunit 1

Entrez Gene IDs

672 (Human); 12189 (Mouse)

Gene Symbol

BRCA1

UniProt

P38398 (Human)

Additional BRCA1 Products

Product Documents for BRCA1 [p Ser1423] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRCA1 [p Ser1423] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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