Bovine IFN-gamma PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2300P

Discontinued Product
IC2300P has been discontinued. View all IFN-gamma products.

Key Product Details

Species Reactivity

Bovine

Applications

Intracellular Staining by Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 345025

View all IFN-gamma Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant bovine IFN-gamma
Gln24-Thr166
Accession # NP_776511

Specificity

Detects bovine IFN-gamma in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with IFN-gamma from equine, canine, or feline systems is observed and no cross-reactivity with human, cotton rat, mouse, porcine, or rat IFN-gamma is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Bovine IFN-gamma PE-conjugated Antibody

Detection of IFN-? antibody in Bovine PBMCs antibody by Flow Cytometry.

Detection of IFN‑ gamma in Bovine PBMCs by Flow Cytometry.

Bovine peripheral blood mononuclear cells were stained with Rat Anti-Bovine IFN-? PE-conjugated Monoclonal Antibody (Catalog # IC2300P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Intracellular Molecules.

Applications for Bovine IFN-gamma PE-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Bovine peripheral blood mononuclear cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IFN-gamma

Interferon-gamma (IFN-gamma), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature bovine IFN-gamma exists as a noncovalently linked homodimer of 20‑25 kDa variably glycosylated subunits (3). It shares 78%‑80% amino acid (aa) sequence identity with canine, feline, equine, and porcine IFN-gamma and 42%‑59% with cotton rat, human, mouse, rat, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells including monocytes, NK cells, gamma delta T cells, CD8+ Tcells, multiple T cell subsets (inlcuding Th1, CCR7- TEM and CD103+CD69+ TRM cells) plus proinflammatory FoxP3 regulatory T cells (6-12). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 13, 14). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (15, 16). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7). Finally, IFN-gamma regulates blood cell production, particularly during immune challenge. In particular, IFN-gamma appears to promote monocyte production while depressing neutrophil, B cell and eosinophil production (17).

References

  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Cerretti, D.P. et al. (1986) J. Immunol. 136:4561.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. Yamaguchi, R. et al. (2015) Blood Cells Mol. Dis. 55:127.
  8. Paolini, R. et al. (2015) Cytokine Growth Factor Rev. 26:113.
  9. Hou, L. et al. (2015) Int. Immunopharmacol. 28:887.
  10. de Graujo-Souza, P.S. et al. (2015) J. Immunol Res. 2015:849573.
  11. Greginat, J. et al. (2014) Front. Immunol. 5:630.
  12. Padiyan, P. and J. Zhu (2015) Cytokine 76:13.
  13. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  14. Zhu, L. et al. (2014) Int. Rev. Immunol. 34:82.
  15. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  16. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
  17. de Bruin, A.M. et al. (2014) Blood 124:2479.

Long Name

Interferon gamma

Alternate Names

IFG, IFI, IFNG, IFNgamma

Entrez Gene IDs

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Gene Symbol

IFNG

UniProt

NP_776511

Additional IFN-gamma Products

Product Documents for Bovine IFN-gamma PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Bovine IFN-gamma PE-conjugated Antibody

For research use only

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.