Blooms Syndrome Protein Blm Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-324
![Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324]](https://resources.bio-techne.com/images/products/Blooms-Syndrome-Protein-Blm-Antibody-Western-Blot-NB100-324-img0003.jpg "Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Novus Biologicals Goat Blooms Syndrome Protein Blm Antibody - BSA Free (NB100-324) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Blooms Syndrome Protein Blm Antibody - BSA Free
Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324]
Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324]](https://resources.bio-techne.com/images/products/Blooms-Syndrome-Protein-Blm-Antibody-Immunoprecipitation-NB100-324-img0005.jpg "Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324]")
Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324]
Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324] - Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-BLM antibody NB100-324 (lot NB100-324-2) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot NB100-324-1) and rabbit anti-BLM antibody NB100-214. For blotting immunoprecipitated BLM, NB100-324 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for Blooms Syndrome Protein Blm Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg of lysate
Western Blot
1:10000 - 1:25000
Application Notes
NB 100-324 may be used for Western Blot and immunoprecipitation. Suggested working dilutions: * WB - 1:1,000-1:10,000 (ECL) IP - 1 to 4 ul/mg of lysate IHC / ICC - Not Determined *The investigator should determine the optimal working dilution for a specific application.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Blooms Syndrome Protein Blm
Long Name
Bloom syndrome protein
Alternate Names
BLM, EC 3.6.4.12, RecQ protein-like 3, RecQ2, RECQL3
Entrez Gene IDs
641 (Human)
Gene Symbol
BLM
UniProt
Additional Blooms Syndrome Protein Blm Products
Product Documents for Blooms Syndrome Protein Blm Antibody - BSA Free
Product Specific Notices for Blooms Syndrome Protein Blm Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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