Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Blooms Syndrome Protein Blm Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Blooms Syndrome Protein Blm Antibody - BSA Free

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-324] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324]

Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324]

Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-324] - Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-BLM antibody NB100-324 (lot NB100-324-2) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot NB100-324-1) and rabbit anti-BLM antibody NB100-214. For blotting immunoprecipitated BLM, NB100-324 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Blooms Syndrome Protein Blm Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg of lysate

Western Blot

1:10000 - 1:25000
Application Notes
NB 100-324 may be used for Western Blot and immunoprecipitation. Suggested working dilutions: * WB - 1:1,000-1:10,000 (ECL) IP - 1 to 4 ul/mg of lysate IHC / ICC - Not Determined *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Blooms Syndrome Protein Blm

The Bloom's syndrome gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DexH-box-containing DNA and RNA helicases.

Long Name

Bloom syndrome protein

Alternate Names

BLM, EC 3.6.4.12, RecQ protein-like 3, RecQ2, RECQL3

Entrez Gene IDs

641 (Human)

Gene Symbol

BLM

UniProt

NP_000048.1 (Human)

Additional Blooms Syndrome Protein Blm Products

Product Documents for Blooms Syndrome Protein Blm Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Blooms Syndrome Protein Blm Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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