Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Blooms Syndrome Protein Blm Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1425 and the C-terminus (residue 1477) of human Bloom Syndrome using the numbering given in entry NP_000048.1 (GeneID 641).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Blooms Syndrome Protein Blm Antibody - BSA Free

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (15 ug) from HEK293T, K-562, Jurkat, HeLa, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214]

Western Blot: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (50 ug) from TCMK-1 and BIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BLM antibody NB100-214 (lot 4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-214]

Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-214]

Immunoprecipitation: Blooms Syndrome Protein Blm Antibody [NB100-214] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody (NB100-214, lot 4) used for IP at 6 ug per reaction. BLM was also immunoprecipitated by a previous lot of this antibody (lot 3). For blotting immunoprecipitated BLM, NB100-214 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for Blooms Syndrome Protein Blm Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 to 10 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Optimal dilutions should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Blooms Syndrome Protein Blm

The Bloom's syndrome gene, BLM, plays an important role in the maintenance of genomic stability in somatic cells. The BLM protein is a 1417 amino acid peptide with homology to the RecQ helicases, a subfamily of DexH-box-containing DNA and RNA helicases.

Long Name

Bloom syndrome protein

Alternate Names

BLM, EC 3.6.4.12, RecQ protein-like 3, RecQ2, RECQL3

Entrez Gene IDs

641 (Human)

Gene Symbol

BLM

UniProt

P54132 (Human)

Additional Blooms Syndrome Protein Blm Products

Product Documents for Blooms Syndrome Protein Blm Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Blooms Syndrome Protein Blm Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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