Beta Ig-h3/TGFBI Antibody (7Q5O9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15994
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7Q5O9 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Beta Ig-h3/TGFBI (Q15582). AVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWAS
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Beta Ig-h3/TGFBI Antibody (7Q5O9)
![Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]](https://resources.bio-techne.com/images/products/Beta-Ig-h3-TGFBI-Antibody-7Q5O9-Immunohistochemistry-Paraffin-NBP3-15994-img0001.jpg "Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]")
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Human esophageal using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]
Analysis of lysates from A549 cells using TGF beta induced (TGFBI) Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. A549 treated with hTGF beta-3 (100 ng/ml) at 37℃ for 16 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 45 s.Applications for Beta Ig-h3/TGFBI Antibody (7Q5O9)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: beta IG-H3
Long Name
TGF-beta Induced Gene H3
Alternate Names
beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP
Gene Symbol
TGFBI
Additional beta IG-H3 Products
Product Documents for Beta Ig-h3/TGFBI Antibody (7Q5O9)
Product Specific Notices for Beta Ig-h3/TGFBI Antibody (7Q5O9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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