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BAAT1 Antibody (JE64-71)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32089

Recombinant Monoclonal Antibody
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NBP3-32089

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE64-71 expressed in HEK293

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant protein within human BAAT1 aa 672-821/821. (Uniprot: Q6PJG6)

Localization

Cytoplasm, Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAAT1 Antibody (JE64-71)

BAAT1 Antibody (JE64-71) Western Blot: BAAT1 Antibody (JE64-71) [NBP3-32089]

Western Blot: BAAT1 Antibody (JE64-71) [NBP3-32089]

Western blot analysis on different lysates with NBP3-32089 at 1/500 dilution. Lane 1: MCF-7 cell lysate Lane 2: PC-3 cell lysate Lane 3: 293 cell lysate Lane 4: K562 cell lysate Lane 5: A431 cell lysate Lane 6: THP-1 cell lysate Lane 7: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 88 kDa Observed band size: 77 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32089 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Applications for BAAT1 Antibody (JE64-71)

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAAT1

BAAT1 (BRCA1-associated protein required for ATM activation-1) was identified by yeast two hybrid analysis. BAAT1 binds to ATM, localizes to double-strand breaks, and is required for Ser1981 phosphorylation of ATM.

Alternate Names

BAAT1, BRCA1-associated ATM activator 1, BRCA1-associated protein required for ATM activation protein 1, C7orf27, chromosome 7 open reading frame 27, HEAT repeat-containing protein C7orf27, MGC22916

Gene Symbol

BRAT1

Additional BAAT1 Products

Product Documents for BAAT1 Antibody (JE64-71)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BAAT1 Antibody (JE64-71)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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