Aurora B Antibody - BSA Free

Western Blot: Aurora B Antibody - BSA Free [NB100-294] -

Database analysis and AURKB expression level in melanoma. (A) Volcano plot of gene expression analysis in GSE 4587. The X-axis indicates the fold change between normal skin or nevi and melanoma samples, and the Y-axis indicates on a log10 scale the p-values obtained from a supervised logistic regression analysis testing the association of gene expression between normal skin or nevi/melanoma. The horizontal dotted lines mark the significance cutoffs (i.e., fold change more than 4 times or less than 0.25, and p-value less than 0.001). (B) Heat map of analysis of the expression of these selected genes in GSE 4587. AURKB has a higher expression level in melanoma compared with non-melanoma (normal skin and nevi). (C) Pathway enrichment analysis of differential gene analysis. The top 2 pathways were selected, fold change more than 4 times, p-value less than 0.0001. (D) The Venn diagram lists the common genes in both pathways. (E) Melanoma patients with high expression of AURKB show a significantly lower overall survival rate. (F)AURKB is highly expressed in melanoma compared with normal skin as determined by GEPIA. (G) AURKB is significantly overexpressed in melanoma tissue compared with normal skin as shown in a tissue array analysis; the scale bar = 100 um. (H) AURKB is overexpressed in drug-resistant melanoma cell lines (A375R, M238R, and M249R) compared with drug-sensitive melanoma cell lines (A375, M238 and M249). Statistical significance was determined by Student's t-test and the asterisks indicate a significant change compared with the control group (*, p < 0.05; ***, p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32863956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Aurora B Antibody - BSA Free [NB100-294] -

AURKB mediates EGF associated BRAF/MEK/ERKs and PI3-K/AKT pathways. (A, B) Knocking down of AURKB suppressed the level of EGF in A375 and A375R tumor from xenograft mice compared with shcontrol. The asterisks indicate a significant difference compared with the shcontrol group (*, p < 0.05; ** p < 0.01). (C) Knocking down of AURKB increased levels of c-PARP, c-caspase 3, and decreased of Bcl-2 level of A375 and A375R cell lines. (D, E) Western blot analysis of activation of BRAF/MEK/ERKs and PI3-K/AKT by knocking down of AURKB in A375 and A375R cell lines. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32863956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Aurora B Antibody - BSA Free [NB100-294] -

HI-511 binds to and effectively suppresses the activation of AURKB and BRAF V600E. (A) The structure of HI-511. (B) Computer docking model of HI-511 binding with AURKB. (C) Computer docking model of HI-511 binding with BRAF V600E. (D) HI-511 inhibits AURKB in vitro. Active kinase AURKB and HI-511 (0, 1, 5 and10 uM) or APIO-EE-9 (5 uM) were mixed with the substrate histone H3. The relative amounts of phosphorylated substrate were visualized by Western blot. (E) HI-511 inhibits BRAF V600E in vitro. Active kinase BRAF V600E and HI-511 (0, 1, 5, 10 uM) or vemurafenib (5 uM) were mixed with the substrate phosphatidylinositol. The relative amounts of phosphorylated substrate were visualized by Western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32863956), licensed under a CC-BY license. Not internally tested by Novus Biologicals.