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ATG9A Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-56893F

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NB110-56893F
Conjugate
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Alligator, Bovine, Chicken, Drosophila, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide made to a region within the C-terminus of human ATG9A (between residues 750-839). .

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 26353861). Use in Alligator reported in scientific literature (PMID:32061056).

Localization

Membrane; multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for ATG9A Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB110-56893. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: ATG9A

ATG9A (autophagy-related protein 9A) is the only transmembrane member identified among the classical ATG proteins family which are essential to autophagy. Yeast ATG9 protein localizes to the pre-autophagosomal structure (PAS) as well as cytoplasmic punctate structures, whereas, the mammalian ATG9A localizes to endocytic compartments, the trans-Golgi network and autophagic membranes. Moreover, ATG9 is recruited to the PAS in an ATG11-dependent manner under nutrient-rich conditions, whereas during starvation, recruitment of ATG9 depends on Atg17p. ATG9A does not steadily reside at one place in cells, but rather dynamically shuttles between a juxta-nuclear trans-Golgi network compartment and late endosomes under autophagic or starvation conditions. Moreover, ATG9A accumulation is upregulated upon starvation in autophagosomes and the starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. ATG9A interacts with SUPT20H and has been proposed to mediate membrane transport to generate autophagosomes.

Alternate Names

APG9 autophagy 9-like 1, APG9 autophagy 9-like 1 (S. cerevisiae), APG9L1APG9-like 1, ATG9 autophagy related 9 homolog A (S. cerevisiae), autophagy 9-like 1 protein, autophagy-related protein 9A, FLJ22169, mATG9, MGD3208

Gene Symbol

ATG9A

Additional ATG9A Products

Product Documents for ATG9A Antibody [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ATG9A Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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