ATG7 Antibody (24GB6075)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51003

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NBP3-51003-100ul
NBP3-51003-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB6075

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ATG7 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human ATG7 (Uniprot: O95352)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ATG7 Antibody (24GB6075)

ATG7 Antibody (24GB6075)

Immunocytochemistry/ Immunofluorescence: ATG7 Antibody (24GB6075)[NBP3-51003] -

Immunocytochemical staining of HT-1080 cells with ATG7 antibody (1:1,000) . Nuclei were stained blue with DAPI; ATG7 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
ATG7 Antibody (24GB6075)

Flow Cytometry: ATG7 Antibody (24GB6075)[NBP3-51003] -

Validation of ATG7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with ATG7 antibody (1:2,000) and analyzed using BD flow cytometer.
ATG7 Antibody (24GB6075)

Flow Cytometry: ATG7 Antibody (24GB6075)[NBP3-51003] -

Flow cytometric analysis of ATG7expression in HT-1080 cells using ATG7 antibody (1:2,000). Green, isotype control; red, ATG7.
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Applications for ATG7 Antibody (24GB6075)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG7

FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. SIMILARITY: Belongs to the ATG7 family.

Long Name

ATG7 Autophagy Related 7 Homolog

Alternate Names

APG7, APG7L, GSA7

Gene Symbol

ATG7

Additional ATG7 Products

Product Documents for ATG7 Antibody (24GB6075)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATG7 Antibody (24GB6075)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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